Journal:

Article Title: Hook3 interacts with PCM1 to regulate pericentriolar material assembly and the timing of neurogenesis

doi: 10.1016/j.neuron.2010.01.011

Figure Lengend Snippet: (A) Western blot of brain lysates from various developmental stages. (B) Immunostaining of E14 brain sections. Hook3 (green) is highly expression in CP and VZ. In the VZ, Hook3 is expressed by nestin-positive neural progenitors. (C) Diagram of en face view of the ventricular surface. (D) En face view of Hook3 expression pattern at the ventricular surface. (E) N2A cells immunostained for Hook3 and pericentrin. The enlarged cell shows that Hook3 localizes to granular structures that concentrate to the centrosome. Scale bars: 50 μm in B; 10 μm in D-E. See also Figure S1.

Article Snippet: Primary antibodies used were anti-Hook3 (kindly provided by H. Kramer), anti-nestin (Rat401, 1:500; BD Pharmingen), anti-Tuj1 (monoclonal, 1:1000, Covance), anti-pericentrin (monoclonal, 1:100, BD Biosciences), anti-phospho histone H3 (polyclonal, 1:500, Upstate), anti-BrdU (monoclonal, 1:500, Dakocytomation), anti-Ki67 (poluclonal, 1:500, Neomarkers), anti-α-tubulin (1:1000, Sigma T5168), anti-PCM1 (polyclonal, 1:500, kindly provided by A. Merdes), anti-GFP (Chicken polyclonal, 1:1000, Aves Labs).

Techniques: Western Blot, Immunostaining, Expressing